Fig. 2
Effect of adenoviral transduction of human amnion epithelial cells on pancreatic marker gene expression. Fold-change expression of human pancreatic marker genes was assayed by means of qPCR (a) 2 days and (b) 7 days after mPdx1 adenoviral transduction of p2 hAECs. Fold-change is relative to untreated cells. Error bars represent mean ± SD. Only those genes with a Cq <35 are represented here. Of the 21 genes assayed, expression of 13 genes on day 2 and 18 genes on day 7 were detected. (c) Five genes in particular- NANOG, SOX17, NGN3, NEUROD1 and PAX4—showed significant increase in expression while RFX6 and RFX3 showed a significant loss of expression over the 7 day period. (* p < 0.05, ** p < 0.005, *** p < 0.0005; n = 4)