Fig. 1

Comparison of average Cq values between untransduced p2 hAECs and crude human islet preparations for pancreatic progenitor-specific genes. Pancreatic progenitor-specific genes were assayed by means of qPCR for untransduced p2 hAECs (baseline gene expression) and crude human islet preparations (positive control). Error bars represent mean ± SD. It can be noted that endogenous CK19 and SOX9 expression is high in untransduced hAECs while expression of PDX1, RFX6, SOX17, FOXA2 and NKX2.2 is virtually absent (Cq > 35—gene expression undetectable, Cq 30–35—low level of gene expression, Cq 25–30—high level of gene expression, Cq <25—very high level of gene expression)