Fig. 2

Akt regulates expression of neoblast and progeny markers. a Gene expression levels for markers of neoblasts (smedwi-1) and neoblast early (Prog-1) and late (AGAT-1) progeny were evaluated with qPCR at days 10, 20 and 30 after first dsRNA injection and represented in fold change relative to each sample's individual control. b Fold change in gene expression levels involving markers of neoblast subpopulations sigma (blue) and zeta (red). The mRNA levels were measured in X1 sorted cells at day 30 after first injection was used to determine gene expression of Smed-AKT(RNAi) animals. All values are relative to equal number (~20,000) of X1 cells sorted from control-water injected animals at 30 days. Gene expressions are all relative to the internal control, the ubiquitously expressed clone H.55.12e. Graphs represent mean ± s.e.m. of triplicated samples of two or more biological replicates with at least ten animals per experiment. P values ** < 0.001, *** < 0.0005 and **** < 0.0001, were calculated by two way ANOVA