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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Computational prediction and experimental validation of novel Hedgehog-responsive enhancers linked to genes of the Hedgehog pathway

Fig. 4

Functional verification of GLI-dependent enhancer activity. Putative regulatory regions were cloned upstream of a minimal promoter co-transfected into C2C12 cells, along with a GLI1 expression vector. Relative activity is plotted (stimulated/basal). The Ptch2 region is used as a positive control. Novel regions annotated to the Boc, Dpp6, Hhip, Hipk2, Ptch1, Scube1, and Tgfbr2 loci exhibit upregulation in response to GLI1 co-transfection (green). Dependence on GLI was assessed by mutating all putative GLI TFBS (GKO) within the sequence and retesting in the assay (gray)

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