Table 2 Characteristics of migratory cell types. See Methods for abbreviations in table

Eleocyte (Fig. 4a and b)

• Large, round, with a globular cluster of granules.

• Found within coelomic cavity, single or in clusters attached to septa, gut, or chaetal sac muscles.

• Can “jump” after detaching from anchor points, or crawl along the peritoneal lining.

37

avg: 12.7

avg: 3.5

avg: 6882

min: 1.30 / 1.30

min: 0.00 / 0.05

rng: 9–19

med: 2.8

med: 1545

1stQ: 3.97 / 2.55

1stQ: 0.00 / 0.33

max: 33.9

max: 122,671

med: 6.99 / 4.63

med: 0.37 / 0.84

avg: 1716 / 5.50

avg: 2578 / 1.08

3rdQ: 951 / 6.99

3rdQ: 1.27 / 1.41

max: 16,210 / 18.64

max: 1.02e5 / 4.98

n: 37 / 25

n: 37 / 25

“Carrier” (Fig. 4c and d)

• Medium size, variable irregular shape containing few to several granules.

• Found within coelom.

• Move by sliding and pseudopodial “walking”.

143

avg: 10.8

avg: 3.3

avg: 5167

min: 0.76 / 0.76

min: 0.00 / 0.03

rng: 7–18

med: 2.5

med: 871

1stQ: 3.75 / 3.04

1stQ: 1e-5 / 0.42

max: 20.1

max: 79,334

med: 6.91 / 4.86

med: 0.50 / 0.79

avg: 801 / 5.60

avg: 1.00 / 1.42

3rdQ: 568 / 7.10

3rdQ: 1.05 / 1.25

max: 14,730 / 48.74

max: 32.8 / 32.8

n: 143 / 101

n: 143 / 101

Amebocyte (Fig. 4e)

• Large amoeboid cells containing usually 1–2 large granules, sometimes more or none.

• Found along the inner side of the body wall

• Crawl mostly on dorsal peritoneum by extending long pseudopodia, stretching as much as 50 μm or more.

90

avg: 17.0

avg: 2.9

avg: 12,007

min: 1.06 / 1.06

min: 0.00 / 0.14

rng: 8–30

med: 1.9

med: 3019

1stQ: 2.03 / 1.92

1stQ: 0.48 / 0.59

max: 33.0

max: 170,254

med: 3.66 / 2.81

med: 0.97 / 1.03

avg: 341 / 3.97

avg: 1.17 / 1.37

3rdQ: 7.16 / 4.92

3rdQ: 1.51 / 1.56

max: 9673 / 22.06

max: 6.35 / 6.35

n: 90 / 77

n: 90 / 77

Hyalinocyte (Fig. 4f and g)

• Medium to large, round or oblong with smooth texture; no granules.

• Typically found along the inner side of the body wall

• No obvious motility structures, swing from an attached edge.

12

avg: 10.7

avg: 4.7

avg: 1518

min: 0.89 / 0.89

min: 8e-6 / 0.02

rng: 8–16

med: 2.7

med: 601

1stQ: 3.27 / 3.01

1stQ: 0.06 / 0.92

max: 11.3

max: 4586

med: 6.20 / 5.87

med: 1.06 / 1.18

avg: 113 / 6.25

avg: 1.11 / 1.34

3rdQ: 11.20 / 7.23

3rdQ: 1.64 / 1.70

max: 743 / 17.93

max: 3.73 / 3.73

n: 12 / 10

n: 12 / 10

“Roller” (Fig. 4h and i)

• Medium size, round cells, with none, one or two granules.

• Found on coelomic cavity, in tangential contact with their substrate or detached.

• Move by rolling over the substrate aided by small filopodia.

94

avg: 8.6

avg: 4.9

avg: 3047

min: 0.87 / 0.87

min: 0.00 / 0.07

rng: 7–12

med: 4.7

med: 1555

1stQ: 5.66 / 5.23

1stQ: 0.28 / 0.57

max: 21.3

max: 24,365

med: 6.85 / 6.47

med: 0.71 / 0.92

avg: 1032 / 6.71

avg: 1.07 / 1.29

3rdQ: 9.42 / 7.45

3rdQ: 1.36 / 1.43

max: 26,140 / 21.27

max: 6.81 / 6.81

n: 94 / 78

n: 94 / 78

“Slider” (Fig. 4j and k)

• Small to medium, spindle shaped cells, with no granules (rarely one).

• Large surface in contact with the substrate, often over ventral nerve cord or lateral peritoneum.

• Move by sliding over the substrate.

115

avg: 9.6

avg: 2.0

avg: 3071

min: 0.56 / 0.55

min: 0.00 / 0.04

rng: 6–16

med: 1.2

med: 887

1stQ: 1.36 / 1.28

1stQ: 0.09 / 0.75

max: 32.3

max: 66,262

med: 2.70 / 1.91

med: 0.86 / 1.22

avg: 432 / 2.57

avg: 1.38 / 1.79

3rdQ: 6.33 / 3.61

3rdQ: 2.17 / 2.48

max: 11,030 / 8.95

max: 6.98 / 6.98

n: 115 / 89

n: 115 / 89