Fig. 4

Six migratory cell populations recognized from analysis of 4D datasets. a-k Images of representative cells of each putative cell type (white arrowheads). Scale bars: 20 μm. a Round eleocytes clinging to a septum and chaetal muscles. b Migrating eleocyte sliding along the dorsal body wall. c Two carrier cells near the ventral surface of the coelom; note the variable morphology and presence of one to many granular inclusions. d A carrier moving along the ventral body wall. e An amebocyte moving along the dorsal body wall; these cells can be distinguished from amoeboid carriers by their larger size and different movement behavior. f A fusiform hyalinocyte, clinging by one end from the dorsal body wall. g A round hyalinocyte with one side attached to the dorsal body wall. h A roller with granular inclusions moving along the dorsal body wall. i A roller without inclusions moving along the dorsal body wall; note the fine filopodia. j A slider moving anteriorly along the peritoneal lining of the lateral body wall. k A slider moving posteriorly along the dorsal surface of the ventral nerve cord. l Vector plots of overall XY plane displacement of individual cells in anterior amputees (green), uncut worms (black), and posterior amputees (red). Each arrow represents the difference between XY coordinates measured at the start and end of the track of one cell (i.e., longer arrows represent larger total displacements); vectors are not corrected for track duration. Scale for all plots is shown at lower left. Shown at right for each vector group are the sample size (n) and the p-value of Wilcoxon rank sum tests for the average X displacement being significantly different from zero (calculated only for groups where n > 10; p = 0* indicates p < 0.001)