Fig. 3

Time-lapse imaging and growth rate analysis during regeneration. a Frames from a time-lapse movie of anterior regeneration using a glass-bottom dish mount. Frames shown are 20 h apart, except for the first two frames shown which are 15 h apart. Dotted outline at 100 hpa illustrates how regenerate area was measured for growth rate analysis. b Frames from a time-lapse movie of posterior regeneration using a glass-bottom dish mount. Frames shown are 10 h apart. Defecation is evident in the 62 hpa frame, indicating the re-opening of the anus. c Frames from a time-lapse movie of anterior regeneration using a slide-and-oil mount. Frames shown are 15 h apart. In a-c, time is shown as hours post-amputation; amputation level is indicated by arrowheads. d Growth curves of anterior regenerate cross-sectional area over time for two different individuals (shown in red and purple).