Fig. 6

Apontic inhibits STAT function via a conserved genetic circuit in testes. a-b, d-e 2-D projections of optical sections of testes stained with antibodies directed against Zfh-1 (magenta), Eya (white), and N-Cad (white, to label hub, outlined in blue). Insets show Zfh-1 expression alone. a A testis from a Socs36E mutant male contains a wild-type number and organization of Zfh-1+ cells. b A single copy reduction of apt function (via null allele apt ¹⁶⁷) in a Socs36E ^EY06665/Socs36E ¹⁷⁸ mutant male significantly expands the Zfh-1+ population in the testis. c Zfh-1+ cell quantification in the specified genotypes shows a genetic interaction between apt and Socs36E. d Testis from a male bearing the UAS-miR-279sponge (miR-279sp) with no Gal4 driver displays a wild-type number and arrangement of Zfh-1+ cells. e The Zfh-1+ population is expanded when the miR-279sponge is expressed via c587-Gal4. f Total Zfh-1+ cell number increases when miR-279sponge is expressed. g Quantification of Zfh-1+ cells in the specified genotypes reveals a genetic interaction between apt and miR-279. For all images the scale bar = 20 μm. For graphs, two-tailed t-tests were used to assess significance where n.s. denotes not significant, *p<0.05, ***p<0.005, ****p<0.0001. Significance was measured between the experimental genotype(s) and apt ¹⁶⁷/+ (c, g) or c587>GFP (f) unless otherwise indicated by a bar. “n” indicates number of testes scored