Fig. 4

Somatic loss of apt expands the CySC population. a-b Projected images of optical sections of testes from males of the indicated genotypes, stained with antibodies directed against BrdU (magenta, to label dividing cells) and Tj (white). DAPI (blue) labels nuclei. The hub is outlined in blue. Scale bar = 20μm. Insets display single optical sections of indicated staining alone. a In control testes, CySCs divide at the hub (arrow). b When apt is reduced in the soma, a Tj+ cell divides several cell diameters away from the hub (arrowhead). c Percentage of testes, of the indicated genotypes, in which BrdU+ somatic cells were observed. Quantification was separated between CySCs (first tier of Tj+ cells around the hub, blue) and Tj+ cells more than one somatic cell tier away from the hub (red). Two-tailed Fisher’s exact tests were performed to analyze significance of the data, where *p<0.05 and **p<0.01. “n” indicates number of testes scored