Fig. 1From: Revisiting in vivo staining with alizarin red S - a valuable approach to analyse zebrafish skeletal mineralization during development and regenerationQuantification of mineral apposition in developing zebrafish larvae. Schematic representation of the quantification of the mineral apposition rates in vertebral centra following ARS or calcein staining. a Mineral apposition was determined (at 24, 48 and 72 h post-staining - hps) by monitoring the mineralized surface areas (SA’s) of the three least mineralized vertebral centra (grey) in the beginning of the experiment. b Centra SA’s were calculated based on width (C.Wi) and height (C.Hi), as indicatedBack to article page