Fig. 4

Validation of miRNAs by real-time RT-PCR. Expression levels of five miRNAs (ssc-miR-103, ssc-miR-107, ssc-miR-127, ssc-miR-133a, and ssc-miR-133b) were detected by real-time RT-PCR and microarray chip. We scaled the raw data from the real-time RT-PCR to make it comparable with the microarray data. Each time point was replicated three times using independently collected samples; the average is shown with the standard deviation. R represents the Pearson correlation coefficient. Expression levels of the five miRNAs were in concordance with the normalized microarray data (R > 0.9). Ssc-miR-103 and ssc-miR-107 expression was slightly lower in premolars (Dpm) than in other types of teeth, ssc-miR-133a and ssc-miR-133b expression was much higher in Dpm than in other types of teeth, and ssc-miR-127 expression gradually increased from the incisor (Di) to the molar (Dm)