Fig. 2

Myotome organization in proximal and distal regenerates starts at a fixed time after amputation, but blastema size differs depending on amputation level. a. Tails were amputated proximally (at the 4th myotome posterior to the cloaca, red) or distally (16th myotome posterior to the cloaca, blue). Scale bar, 5 mm. b. Regenerating tails were monitored over time and the number of visible myotomes was scored. c. Fluorescent and brightfield images of regenerating tail depicting the parameters measured in Fig. 2d and Fig. 3. Scale bar, 1 mm. d. The length of regenerated tissue posterior to the last myotome (called blastema) was measured until the tail tip (denoted L1 in Fig. 2c) as a function of time after amputation. Values shown at each time point are the mean of at least six animals; error bars represent standard deviations from the mean. Results shown are representative of two independent experiments