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Fig. 9 | BMC Developmental Biology

Fig. 9

From: Hsp90 and hepatobiliary transformation during sea lamprey metamorphosis

Fig. 9

Hsp90 siRNA treatment decreased CK19 and BCL2 immunofluorescence in sea lamprey liver during metamorphosis. Confocal images showing the biliary tree in sea lamprey liver (whole organ processed with a modified CLARITY method [73]) during metamorphosis. Left panels are taken from the anterior, and the right panels from the posterior end. Images of liver cell surface marker cytokeratin 19 (1:100 mouse-anti-CK19, Thermo Fisher; stained with 2 μg/ml Alexa Fluor 350-goat-anti-mouse IgG, Life Technologies; blue fluorescence) and anti-apoptotic marker BCL2 (1:100 rabbit-anti-Bcl2, Santa Cruz; stained with 2 μg/ml Alexa Fluor 488-donkey-anti-rabbit IgG, Life Technologies; green fluorescence) are superimposed. White arrows indicate the biliary tree. Yellow arrows indicate the dead cells and surrounding canaliculi and ductules in the liver. Note that the biliary tree was mostly degenerated in hsp90 siRNA-treated liver. Scale bar: 500 μm

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