Fig. 4

Comparative transcriptome of ICM and TE obtained from in vitro vs. in vivo blastocysts. Transcriptome profile of ICM and TE derived from in vivo generated blastocysts in this study (VIVO) was compared with the in vitro (VITRO) studies of Ozawa et al. [2012] (left panel) and Nagatomo et al. [20] (right panel). A and A’) differential expression with list of important genes misregulated in vitro studies compared to our in vivo study. The colored symbols below the genes lists represent genes that were upregulated (red upward triangle), downregulated (green downward triangle) or similarly (gray circles) expressed in the ICM of vitro (left symbols) vs. VIVO (right symbols) transcriptome studies. B and B’) The overlap between transcriptional profile of in vitro (blue lines) and in vivo (red lines). C and C’) The most important canonical pathways emerged from genes found to be misregulated in ICM and TE transcriptomes of vitro vs. in vivo studies. D and D’) Gene ontology analysis of from genes found to be misregulated in ICM and TE transcriptomes of vitro vs. in vivo studies