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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation

Fig. 2

Characterization of cell proliferation in early and late blastemas. a Fluorescent images obtained on 10uM transverse sections of 7 day (early) and 15 day (late) blastemas that had been stained with DAPI (blue) for nuclei and Edu (green) for dividing cells. Animals were injected with Edu 3 h before tissue was harvested. White lines indicate the boundaries of each region that was quantified (100 uM segments for 7D samples, and 200 uM segments for 15D samples). b Histogram of the labeling index of digital sections from the apical (left) to basal (right) region of each type of blastema. The labeling index was quantified in 4 complete blastema sections for each sample. Error bars are the SEM, students t-test was used to determine statistically significant differences (p < 0.05) in the labeling index of the most basal blastema data-points and the stump data-points (red-filled symbols)

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