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Fig. 5 | BMC Developmental Biology

Fig. 5

From: Meis2 is essential for cranial and cardiac neural crest development

Fig. 5

Heart defects upon conditional inactivation of Meis2 in NCC. (a-a’) Lineage tracing of NCC in AP2α-Cre /Meis2 cKO/ROSA26 embryos at E10.5 (a’) and controls (a). (b-b’) Sections of embryos shown in (a) at the level of PA2 and OFT, compare position and number of β-galactosidase-positive cells in the control and the mutant cKO (arrows in B’). (c-c’) Immunohistochemical staining of Meis2 in cKO (c’) shows the areas of its deletion. (d-d’) Cryosetions of the OFT from AP2α-Cre /Meis2 cKO/ROSA26 stained for β-galactosidase and Sox9, note cells disorganization in cKO (d’). (e-e’) Paraffin-embedded sections of E12 heart stained with anti-Sox9 defective aortic valve in cKO (e’). (f-f’) Meis2 and β-galactosidase double-labeling of E13 OFT shows a number of Meis2-positive cells in cKO (f’) that are excluded from Cre recombined areas. The pulmonary valve does not develop properly. (g-g’) anti-Sox9 immunohistochemical labeling of severed valves in cKO at E14 (g’). Ao, aorta; av, aortic valve; OFT, outflow tract; PA2, pharyngeal arch 2; pa, pulmonary artery, DORV, double outlet right ventricle

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