Table 5 Microscope settings and expected photon counts for the second scan
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Fluorophor/staining | Camera | EMCCD gain | Exposure | Laser | Initial photon count |
|---|---|---|---|---|---|
Alexa | EMCCD 5Â MHz | 2800-3300 | 30Â ms | 10Â % | 17000 |
DAPI | CCD 5 MHz | - | 50 ms-120 ms (lower is better) | 100 % | 8000 – 16000 |