Table 4 Microscope settings and expected photon counts for the first scan
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Fluorophor/staining | Mode | Exposure | Laser | Initial photon count |
|---|---|---|---|---|
Alexa | CCD 5Â MHz | 25Â ms | 50Â % | 17000 |
DAPI | CCD 5Â MHz | 50Â ms-120Â ms (lower is better) | 100Â % | 8000 - 16000 |