Table 3 Optimal refractive indices of oils per stage based on empirical testing
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Stage/cell type | Optimal refractive index of oil |
|---|---|
Zygote (centrally located pronuclei) | >1.534 |
2-cell | 1.528–1.534 |
4-cell | 1.526–1.528 |
8-cell | 1.518–1.526 |
16-cell, morula and blastocyst | 1.512–1.518 |
Spermatozoa, fibroblasts and cell lines | 1.512–1.514 |