Table 1 Examples of primary antibodies used successfully with 3D-SIM
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Host | Target | Official name | Dilution | Company | ID |
|---|---|---|---|---|---|
Goat | Epitope at C-terminus of Lamin B1. Detecting Lamin B1 and, to a lesser extent, Lamin B2 | Lamin B Antibody (M-20) | 1:100 | Santa Cruz | SC-6217 |
Mouse | NUP153 | Anti-NUP153 [QE5] antibody | 1:200 | Abcam | ab24700 |
Rabbit | H3K4me3 | Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade | 1:100 | Abcam | ab8580 |
Mouse | H3K9me3 | Histone H3K9me3 antibody (mAb) | 1:100 | Active Motif | 39285/6 |
Rat | RNAPII-S5p | Monoclonal antibody against CTD phosphoserine epitope 5 | 1:20 | Gift from Dirk Eick (see Markaki et al. [23]) | |
Mouse | B23 | Monoclonal Anti-B23 antibody produced in mouse | 1:1000 | Sigma | B0556 |