Fig. 5
From: 3D structured illumination microscopy of mammalian embryos and spermatozoa
Effect of oils with different refractive indices. The same nucleus stained for lamin B from a rabbit 8-cell stage in vivo embryo scanned with 1.520 (a), 1.526 (b) and 1.530 (c) oils. Note the movement of the optimal focal section away from the cover glass with higher oils. The sides of the orthogonal views are color-coded for high quality sections at around the optimal focal section (green), medium quality sections further away (yellow) and low quality sections even further away (red)