Fig. 3

Inhibition of miR-21 up-regulation after renal damage leads to a delay in tubular regeneration. a In situ hybridization of control und antimiR-21 treated kidney samples after administration of gentamicin. White arrows indicate miR-21 positive tubules in control kidneys. Scale bar: 20 μm b Quantification of miR-21 expression by qPCR in control and antimiR-21 treated samples after administration of gentamicin. Hs-RNU1A-11 was used for normalization. c Comparison of renal recovery of control and antimiR-21 treated kidneys after damage. Dextran-FITC positive kidneys were count and related to number of all kidneys in the respective groups. n = 12 fish/time point d Kinetics of proliferation after kidney damage. Proliferation was measured by injection of EdU 2 h before fish were sacrificed and number of EdU positive tubules on sections was counted for every sample. n = 4 fish/time point e Kinetics of apoptosis after kidney damage. Apoptosis was determined by counting TUNEL-positive tubules, which were related to total number of tubules. n = 4 fish/time point, student’s t-test was used: *p < 0.05, **p < 0.01