Fig. 6

Schematic representation of myogenic and non-myogenic cells in muscles targeted with limb somite or lateral plate electroporation with stable vectors producing reporter genes under the control of different promoters. a Representation of the somitic- (red) and lateral-plate-derived (green) lineages in limb skeletal muscles. Skeletal muscles are composed of myogenic cells at different steps of differentiation somitic-derived (red) and of muscle connective tissue cells lateral plate derived (green). At a specific time point, muscle progenitors, mononucleated muscle cells or myoblasts and differentiated multinucleated muscle cells coexist in muscles. Myogenic cells originate from limb somites (b–d), while connective tissue cells derive from limb lateral plate (e). Limb somite electroporation with stable vectors containing different promoters. b Limb somite electroporation with a stable vector containing a generic CMV/β-actin promoter drives reporter gene expression in myogenic cells at all steps of muscle differentiation. c Limb somite electroporation with a stable vector containing a p57 regulatory element and the chick β-actin promoter drives reporter gene expression in myoblasts and differentiated muscle cells. d Limb somite electroporation with a stable vector containing a MLC promoter drives reporter gene expression in differentiated muscle cells. e Lateral plate electroporation with a stable vector containing a CMV/β-actin promoter drives reporter gene expression in muscle connective tissue cells (inside muscle and surrounding muscle) and tendons