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Fig. 5 | BMC Developmental Biology

Fig. 5

From: A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice

Fig. 5

Gata6 H2B-Venus reporter is expressed in nascent endoderm and mesoderm during gastrulation. a Wholemount immunofluorescence for endogenous GATA6 protein (red) on Gata6 H2B-Venus/+ embryos at early streak (ES, E6.75) and mid-streak (MS, E7.0). Venus is expressed in a mosaic pattern in the VE. The PS region is indicated in the posterior portion of the embryo (bracket). Transverse sections through the PS at E7.0 (level of section indicated by dotted line) are shown below. GATA6 and Venus are expressed in cells that have ingressed from the PS and are migrating laterally to the anterior of the embryo. Venus and GATA6 are not expressed in the Epi, weakly expressed in the VE, and heterogenously expressed in the intervening mesodermal wings (Mes). The red fluorescent signal in the apical VE that forms a ring around the tissue section is likely due to non-specific binding of the GATA6 antibody to the surface of the extraembyonic tissue. b PS explants to track movements of migrating Venus + cells in vitro. The PS region was dissected from the posterior portion of E6.5 Gata6 H2B-Venus/+ embryos and germ layers were separated by enzymatic treatment. The PS was plated on FIBRONECTIN-coated glass chamber slides. The PS explants (grey) attach to the glass and mesenchymal cells migrate outwards to form a mesodermal sheet (green) over the course of several days. Direction of migration is indicated by red dotted arrows. Right-to-left movement (red arrow) of Venus + cells is indicated as they move away from a Gata6 H2B-Venus/+ PS explant over the course of 3 h. Images were captured every 7 min. Proximal (Pr), distal (D), anterior (A), posterior (P), right (R), left (L), primitive streak (PS), mid streak (MS). Nuclei are stained with Hoechst (blue)

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