Conservation analysis of sequences flanking the testis-determining gene Sry in 17 mammalian species

Table 3 De novo motifs in Sry flanking sequences have potential to bind known transcription factors

Start Position	End Position	Sequence	Score	q-value (conservation)	Database of best match	Top-ranked motif ID (based on q-value)	Factor binding motif	q-value (motif scan)
Human Ref
−132	−121	GGGCGGAGAAAT	13.71	0.01	JASPAR	MA0079.2	SP1	9x10⁻³
−204	−193	TTTGCTTGAGAA	10.26	0.02	JASPAR	MA0142.1	POU5F1	3x10⁻³
−1 526	−1 515	TTTTCAAGGTTC	9.44	0.02	JASPAR	MA0017.1	NR2F1	1x10⁻³
−5 152	−5 141	AAAGTGACCTTC	7.80	0.08	Jolma	ESRRG_full_3	ESRRG	1x10⁻³
Mouse Ref
−467	−456	GAAAAAGCGATA	12.16	0.01	Jolma	ONECUT2_DBD	ONECUT2	6x10⁻³
−425	−418	TAACATTC	9.47	0.05	Jolma	HSF1_full	HSF1	3x10⁻²

Conserved sequences identified by WeederH (q-value < 0.1) using either human or mouse as a reference. Significance of scores provided by WeederH was assessed by comparing actual scores to scores obtained from 100 random shufflings of the non-reference sequences (see Methods). Sequences shown here were extended by an additional five nucleotides on both ends from the relevant genomic position before being scanned with FIMO. The top-ranking motif for each sequence is reported here regardless of the q-value reported by FIMO. POUF51 is also known as OCT4. NR2F1 is also known as COUP-TF1

ISSN: 1471-213X