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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Cooperative and independent functions of FGF and Wnt signaling during early inner ear development

Fig. 4

Wnt reporter activity in Spry1 −/− ; Spry2 −/− mutant and control embryos at OEPD and otic placode stages. a, b Wnt reporter activity in Spry1 −/+ ; Spry2 −/+ control and Spry1 −/− ; Spry2 −/− mutant embryos. The OEPD region is bracketed; mid-hindbrain region, (mh). Lateral views of whole-mount embryos are shown. c Wnt reporter activity in a Spry1 −/+ ; Spry2 −/+ control embryo. The otic placode is outlined (white dots). The plane of section shown in (d) is indicated with a white line. d Transverse section through the otic placode in a Spry1 −/+ ; Spry2 −/+ control embryo. An example of the location from which LacZ+ placode lengths were measured is shown with a yellow line. An example of the location from which LacZ- placode lengths were measured is shown with a purple line. Total placode lengths represent the sum of LacZ+ and LacZ- length measurements. e Average fold basal area difference between Spry1 −/+ ; Spry2 −/+ control and Spry1 −/− ; Spry2 −/− mutant embryos at 10 – 13 s. Basal area for each placode was calculated as the sum of length measurements multiplied by the thickness of each section. Individual area measurements were normalized by the average area measurement in Spry1 −/+ ; Spry2 −/+ controls. *, p < 0.05; ** p < 0.001. f Graphical representation of medial-to-lateral total placode, LacZ+, and LacZ- lengths of the same embryos represented in (e). Measurements for each individual otic placode are shown: total placode lengths are shown in grey, LacZ+ lengths in light blue, and LacZ- lengths in light purple. For each genotype, measurements from individual otic placodes were aligned by the maximal total placode length, represented by “0” on the x-axis. The average total placode length, LacZ- length, and LacZ- length are shown with a darker line. Scale bar (ac), 100 μm

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