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Fig. 4 | BMC Developmental Biology

Fig. 4

From: Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells

Fig. 4

Differentiation of Sox7-null ES cells by EB formation. (a-c) Gross morphology of EBs at day 5 in each genotype indicated. Scale bar in C is 200 μm. (d-f) EBs in (a-c) were sectioned and stained with hematoxilyn and eosin. Scale bars are 100 μm. (g) Time course qPCR analysis of Sox7 expression in Sox7-null clones (N4 and N7) and their parental heterozygote line (mt1) duirng EB formation. The relative expression levels normalized to Gapdh were shown with the error bar for standard deviation (n = 3). (h) qPCR analysis of gene expression in EBs at day 6. The relative expression levels normalized to Gapdh were shown with the error bar for standard deviation (n = 3). VE genes are marked in orange and PE genes are marked in blue. (i-k) VE fractions of EBs at day 7 were analyzed by FACS. Dead cells were eliminated by PI staining and living cells were separated by the staining with anti EpCam-PE and anti DppIV-APC. Double-positive fraction was indicated with its proportion (%). (l) qPCR analysis of VE fractions collected by FACS. Relative expression levels to S7mt1 parental line are presented. Expression levels were normalized to Gapdh with the error bar for standard deviation (n = 3)

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