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Fig. 2 | BMC Developmental Biology

Fig. 2

From: Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos

Fig. 2

Optimal number of reference miRNAs. Pairwise variation after successive inclusion of candidate reference miRNAs with lower stability using geNorm provided the optimal combination of miRNAs for normalization purposes. a In bovine oocytes and embryos, the use of two miRNAs was optimal. b In porcine oocytes and COCs the combination of three miRNAs was optimal, although the difference between using 2 or 3 miRNAs was negligible

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