Fig. 2From: Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryosOptimal number of reference miRNAs. Pairwise variation after successive inclusion of candidate reference miRNAs with lower stability using geNorm provided the optimal combination of miRNAs for normalization purposes. a In bovine oocytes and embryos, the use of two miRNAs was optimal. b In porcine oocytes and COCs the combination of three miRNAs was optimal, although the difference between using 2 or 3 miRNAs was negligibleBack to article page