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Fig. 14 | BMC Developmental Biology

Fig. 14

From: Mantle margin morphogenesis in Nodipecten nodosus (Mollusca: Bivalvia): new insights into the development and the roles of bivalve pallial folds

Fig. 14

Mantle margin of adult Nodipecten nodosus. Mantle margin as revealed by magnifying lens (a and b, ventral view, anterior to the left; c, posterior view, dorsal to the top) and histological cross sections (d–i). a Live specimen with valves slightly opened, showing the pallial tentacles and eyes. b Detail of the mantle margin in an anesthetized specimen, showing the large inner mantle fold with tentacles and the middle fold with tentacles and eyes. c Detail of the mantle margin at the auricular region where the inner folds from both mantle lobes are fused. d Adult pallial musculature composed of radial bundles (divided into outer and inner groups) and transverse bundles (arrow). Ventral to the top. Toluidine blue and basic fuchsin (TB). e Long pallial nerve connecting to the circumpallial nerve, the cortical region and the neuropil of which are shown in detail in the inset. Ventral to the top. TB. f Inner mantle epithelium with cells bearing tufts of cilia (arrows). Hematoxylin and eosin (HE). g Detail of neutral mucopolysaccharide secretion (magenta) in a gland cell from the inner mantle epithelium. Periodic acid-Schiff stain (PAS). h Outer mantle epithelium with long microvilli (arrows) covering the surface. HE. i Subepithelial secretory cells with acid mucopolysaccharide content (light blue) in the outer mantle epithelium. Alcian blue (AB). Abbreviations: cn, circumpallial nerve; co, cortex; if, inner mantle fold; ir, inner radial muscles; it, inner fold tentacle; ma, mantle; ne, neuropil; of, outer mantle fold; or, outer radial muscles; pa, pallial eye; pn, pallial nerve; te, middle fold tentacle

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