Fig. 3

RNA interference-mediated Myc knockdown. a Myc expression in the regenerating radial nerve cord on day 2 after injury/DsiRNA injection as determined by qRT-PCR. Two DsiRNA constructs were used, Myc Dsi1 and Myc Dsi2, as described in Methods. Expression values are plotted as fold change relative to a negative control (a GFP-targeting DsiRNA) and expressed in a log2 scale. Error bars show standard deviation. ** p<0.01, *** p<0.001. b-c’ Representative in situ hybridization micrographs showing Myc expression in the radial nerve cord on day 2 after transection/DsiRNA injection. The upper (b and b’) and lower (c and c’) rows of micrographs show longitudinal sections of the RNC of an animal treated with a control (GFP-targeting) DsiRNA and an animal injected with one of the Myc-targeting DsiRNAs (Myc Dsi1), respectively. The micrographs on the right (b’ and c’) are high-magnification view of the boxed regions in the main micrographs on the left (b and c, respectively). The red dashed line indicates the position of the plane of injury. Note the absence of in situ hybridization signal from the cell bodies in the apical region of the ectoneural neuroepithelium of the RNC in the animal treated with Myc Dsi1, but not in the animal, which received the control DsiRNA injection