Figure 1
From: Cropped, Drosophila transcription factor AP-4, controls tracheal terminal branching and cell growth
The tracheal phenotypes of the cropped mutant compared with the blistered/pruned mutant. (A) A pair of dorsal branches (DB, arrowheads) in a wild-type third-instar larva. The air-filled terminal branches ramify extensively on the dorsal muscles. (B) The same view of a blistered ex84 homozygote. Note the absence of terminal branches beyond the arrowheads where the nuclei of terminal cells are. (C) A crp k10415 homozygote. Note the absence of terminal branches beyond the arrowheads, as in the blistered mutant. (D) A pair of lateral trunk LG branches (arrowheads) in a wild-type third-instar larva. (E) The same view of a blistered ex84 homozygote. Note the absence of terminal branches beyond the arrowheads. (F) A crp k10415; btl-Gal4/UAS-GFP larva. (G) A fluorescence image of (F) showing the GFP-labeled tracheal cell cytoplasmic extensions. (H) A dorsal branch of a stage 16/17 wild-type embryo stained with mAb 2A12 to show the tracheal lumen. The arrowhead indicates the position of the terminal nucleus. The dashed line shows the continuation of the base of the dorsal branch out of the plane of focus. (I) The same view of a blistered ex84 mutant. (J) A crp k10415 mutant. The scale bars for A-D = 30 μm, for E-J = 10 μm.