Our WMISH protocol is suitable for fluorescent signal detection. The expression of COE
(B) and beta tubulin
(C and D) in larvae treated with 0.5% SDS was detected using the fluorescent substrate Fast Red (A-D). Panel E is a scanning electron micrograph of a 62 hours post first cleavage (hpfc) old larva with the approximate localisation of the COE expression indicated in red. Panel F is a lateral perspective of a 90 hpfc old larva with indicated engrailed expression. Panels G and H show the positions of beta tubulin expression in a 57 hpfc old larva (G, ventral perspective) and a 100 hpfc old larva (H, lateral perspective). Panels B-D are projections of confocal laser scanning micrographs. Panels B, D and H are reflected about the vertical axis for consistency of presentation. All scale bars are 50 μm.