Thapsigargin-induced changes in Ca
signals. (a) Time course of the appearance of rhodamine 123 in the wing tissues after injection into the abdomen. A single ROI was set for quantitation of intensity changes. The scale bar represents 400 μm. (b) High magnification of the ROI indicated in a at 15 min. Epithelial cells are identified. The scale bar represents 50 μm. (c) Intensity changes over time in the ROI. The changes shown in a are graphically displayed by the pink curve. The other curves of different colors were recorded from different individuals in the same manner. (d) An area of the hindwing around the prospective discal spot (i.e., the basal region). Three ROIs were defined for analysis, which are depicted in e. Thapsigargin was applied at 0 min. At approximately 7 min after injection of thapsigargin, intracellular Ca2+ increased dramatically. The scale bar represents 300 μm. See also Additional file 7: Movie S7. (e) Fluorescence changes over time. The oscillations before the injection of thapsigargin were abolished, and the Ca2+ signals increased suddenly. Thapsigargin injection point is indicated by an arrowhead. (f-h) Tungstate, heparin, and dimethyl sulfoxide (DMSO) injections resulted in no changes in oscillations and Ca2+ levels. Injection point is indicated by an arrow in each panel.