Figure 6

Neurogenic differentiation of STRO-1 ⁺ /c-Kit ⁺ /CD34 ⁻ hDPSCs and STRO-1 ⁺ /c-Kit ⁺ /CD34 ⁺ hDPSCs. A: Confocal analysis of neurogenic differentiation of STRO-1⁺/c-Kit⁺/CD34⁻ hDPSCs and STRO-1⁺/c-Kit⁺/CD34⁺ hDPSCs after 1 week of induction. Immunofluorescent staining show differentiated STRO-1⁺/c-Kit⁺/CD34- hDPSCs and STRO-1⁺/c-Kit⁺/CD34⁺ hDPSCs expressing β-III-Tubulin, MAP2, NeuN, Synapsin and GFAP. Bar: 10 μm. B: Western blot (WB) analysis of β-III-Tubulin, MAP2 and GFAP expression in whole cell lysates of differentiated STRO-1⁺/c-Kit⁺/CD34⁻ hDPSCs and STRO-1⁺/c-Kit⁺/CD34⁺ hDPSCs. Actin bands were used as control of protein loading.