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Figure 5 | BMC Developmental Biology

Figure 5

From: Human Dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations

Figure 5

Evaluation of myogenic differentiation and adipogenic differentiation in STRO-1 + /c-Kit + /CD34 hDPSCs and STRO-1 + /c-Kit + /CD34 + hDPSCs. A: Confocal analysis of myogenic differentiation of STRO-1+/c-Kit+/CD34 hDPSCs and STRO-1+/c-Kit+/CD34+ hDPSCs directly co-cultured with C2C12 mouse myoblasts. Double immunofluorescence labeling shows signals from anti-hMit (green) and anti-myosin (red) and from anti-hNu (green) and anti-myosin (red), respectively. DAPI staining is shown in blue. Bar: 10 μm. B: Evaluation of adipogenic commitment in STRO-1+/c-Kit+/CD34 hDPSCs and STRO-1+/c-Kit+/CD34+ hDPSCs. On the top, histological staining with Oil Red O showing adipogenic differentiation of STRO-1+/c-Kit+/CD34 hDPSCs and STRO-1+/c-Kit+/CD34+ hDPSCs. Cells were counterstained with haematoxylin. Bar: 10 μm. Below, AdipoRed assay performed on both hDPSCs subpopulations, evaluated for lipid droplets formation by confocal microscopy analysis. Differentiated STRO-1+/c-Kit+/CD34 hDPSCs and STRO-1+/c-Kit+/CD34+ hDPSCs show a green fluorescent staining. Bar: 10 μm. At the bottom, quantification of triglycerides (RFU, relative fluorescent unit) carried out by fluorimeter analysis. ***p<0,001 diff STRO-1+/c-Kit+/CD34 hDPSCs vs undiff STRO-1+/c-Kit+/CD34 hDPSCs; §§§ p<0,001 diff STRO-1+/c-Kit+/CD34+ hDPSCs vs undiff STRO-1+/c-Kit+/CD34+ hDPSCs, ANOVA test followed by Newman-Keuls post-hoc test.

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