Inhibition of Musashi phosphorylation promotes cell survival. (A) NSPCs and (B) SH-SY5Y cells were transfected with the eGFP tagged Musashi proteins described in Figure 3 and fluorescent cells were photographed and counted 1 day after transfection then maintained under conditions of serum deprivation for 14 days (NSPC) or 4 days (SH-SY5Y) and counted relative to cell expressing the control, eGFP tag alone. Histogram represents the mean of the three experiments with SEM. The difference in surviving cell numbers were significant (**,p < 0.01 for NSPCs; ****,p < 0.0001 for SH-SY5Y cells). (C) SH-SY5Y cells were maintained as neurospheres in control proliferation media (Con) or plated on adherent substrate under conditions of serum starvation for 3 days (SS). Protein lysates were processed for western blotting with the indicated antibodies.