Figure 1

Musashi undergoes regulatory phosphorylation in response to neural differentiation. (A) Primary embryonic rat neural stem/progenitor cells (NSPCs) were maintained as neurospheres in proliferation media (prolif) or plated on adherent substrate in differentiation media for 2 hrs (diff). Protein lysates were processed for western blotting with the indicated antibodies. (B) Human neuroblastoma SH-SY5Y cells were maintained as neurospheres in proliferation media (prolif), or under differentiation conditions (diff) for the indicated times and analyzed by western blotting with the indicated antibodies.