Figure 7
From: Role of marsupial tammar wallaby milk in lung maturation of pouch young
Bright field images of 3D embryonic lung mesenchymal explants cultured for 3 days in matrix. (A-D) Explants cultured in media with day 20 milk showed cell clumping with no significant changes over time. (E-H) Explants cultured in the presence of day 60 milk showed an increase in cell number and cell differentiation. (I-L) Explants cultured in the presence of day 120 milk showed cell shrinkage and apparent necrosis and a large reduction in cell population. (M-P) Explants cultured as controls in media but in the absence of milk. (Q-T) Immunofluorescence of embryonic lung mesenchymal explants in matrix culture. (Q) Explants cultured in the presence of day 60 milk and stained with DAPI (blue) showed increased cell number and cell differentiation. (T) Cells shown in (Q) at higher magnification. (R) Explants cultured with day 120 milk. (S) Explants cultured as controls in the absence of milk. The cell debris and large reduction in cell population was similar in explants cultured with day 120 milk and control explants. Scale bar 200 μm.