Figure 4From: Role of marsupial tammar wallaby milk in lung maturation of pouch young Expression of lung developmental marker genes in mouse embryonic lung cultured with tammar milk. RNA was isolated from embryonic lungs cultured for 84 h to observe the expression of (A) Sp-C & (B) Sp-B (type-II cell marker gene), (C) wnt-7b, (D) BMP4 and (E) Id-2 marker genes. (A-E) qRT-PCR of Sp-C, Sp-B, wnt-7b, BMP-4 and Id-2 mRNAs were increased in embryonic lung cultured with tammar milk with statistically significant P values (<0.05) shown with an asterisk. Immunohistochemical analysis of (F-I) Sp-C and (J-M) Sp-B in cultured embryonic lung. Lung sections from embryonic lung treated with tammar milk day 20 (F,J), day 60 (G,K), day 120 (H,L) and control (I,M) were immunostained with type-II cell marker surfactant protein C and DAB was used for visualization. (G,K) The Sp-C protein is observed in high concentration in embryonic lungs treated with day 60 milk. (I,M) In control lungs Sp-C protein is detected at low levels. Scale bar 100 μm.Back to article page