Expression of lung developmental marker genes in mouse embryonic lung cultured with tammar milk. RNA was isolated from embryonic lungs cultured for 84 h to observe the expression of (A)
Sp-C & (B)
Sp-B (type-II cell marker gene), (C)
BMP4 and (E)
Id-2 marker genes. (A-E) qRT-PCR of Sp-C, Sp-B, wnt-7b, BMP-4 and Id-2 mRNAs were increased in embryonic lung cultured with tammar milk with statistically significant P values (<0.05) shown with an asterisk. Immunohistochemical analysis of (F-I) Sp-C and (J-M) Sp-B in cultured embryonic lung. Lung sections from embryonic lung treated with tammar milk day 20 (F,J), day 60 (G,K), day 120 (H,L) and control (I,M) were immunostained with type-II cell marker surfactant protein C and DAB was used for visualization. (G,K) The Sp-C protein is observed in high concentration in embryonic lungs treated with day 60 milk. (I,M) In control lungs Sp-C protein is detected at low levels. Scale bar 100 μm.