Effect of tammar wallaby milk (day 60) on branching morphogenesis in cultured mouse embryonic lung. Mouse E 12 lungs treated with tammar milk day 60 (A-D) or under control conditions (E-H) at (A and E) 0 hr (B and F) 24 hrs, (C and G) 48 hrs and (D and H) 72 hrs. (I,J) Increased branching morphogenesis shown by outlining terminal end buds. (K) Quantitation of branching morphogenesis at 0, 24, 48 and 72 hrs shows number of terminal end buds of tammar day 60 milk treated is greater than control treated lungs. Expression of critical developmental marker genes (L) Sp-B & (M) Sp-C (Type-II pneumocytes marker genes), (N) wnt-7b, (O) BMP4 and (P) Id-2 in mouse embryonic lung cultured for 72 h with or without day 60 tammar milk. Scale bar 250 μm. P values (<0.05) are shown with an asterisk.