Table 1 Parameters measured by MaggotTracker

Body Length

mm

The length of the midline of the larva measured using all frames.

Body Length Contracted

mm

Body length measured using a subset of frames when the larval length reaches the local minimum during a stride. ¹

Body Length Extended

mm

Body length measured using a subset of frames where the larval length reaches the local maximum during a stride. ¹

Time Head Bending

%

Percentage of time when the larva has a head angle of over 45 degrees. ^{2, 3}

Time Body Bending

%

Percentage of time when the larva has a body angle of over 45 degrees. ^{2, 3}

Time Bending

%

Percentage of time when the larva has either its head or body angle over 45 degrees. ^{2, 3}

Speed

mm/sec

The positional change of the center point over time. ^{2, 4}

Time Striding

%

Percentage of time when the larva is striding. ^{2, 3}

Speed Striding

mm/sec

Speed measured using a subset of frames when the animal is striding. ^{1, 2, 4}

Stride Duration

sec

Time duration of one stride. ^{1, 2}

Stride Distance

mm

Distance traveled by the center point during one stride. ^{1, 2, 4}

Contraction Rate

mm/sec

The rate of body length change during the contraction phase of a stride. ^{1, 2}

Extension Rate

mm/sec

The rate of body length change during the extension phase of a stride. ^{1, 2}

Stride Count

counts/min

Total number of strides over the video length. ³

Run Distance

mm

Average distance traveled in a run. A run is defined as a period when the animal is striding continuously. ³

Run Duration

sec

Average time duration of a run. ³

Run Stride Count

counts/min

Average number of strides of a run. ³

Run Count

counts/min

Total number of runs over the video length. ³

Distance

mm/min

Total distance traveled by the center point over the video length. ³

Direction Change

%

Percentage points on the track where the animal is changing its direction. ³

Time Inside

%

Percentage of time the animal is away from the plastic ring. ³