Figure 5

Overview of DMR methylation at imprinted genes and expression of associated transcripts in pre-implantation bovine embryos. Schematic representation of DNA methylation imprint dynamics following the blastocyst stage of bovine embryogenesis. Shaded grey area denotes the range of observed methylation values at imprinted loci during pre-implantation embryo development. Solid and dashed lines represents expression profiles of key genes associated with imprinted establishment and maintenance; blue dashed line DNMT3A and DNMT3B, solid orange line DNMT1 and TRIM28/KAP1 and solid orange-dashed orange line ZFP57, UHRF1 family and TET family transcripts. Question marks to the left of the vertical dashed red line denote that technical variation cannot be completely excluded at this stage.