Figure 3

Embryonic imprints at Day 17 and Day 25 methylation patterns resemble those observed in adult somatic tissues. To investigate whether DNA methylation imprints remained stable, levels for each gene (SNRPN, MEST, IGF2R, PLAGL1, PEG10 and H19) were analysed in several adult somatic tissues (n = 1 for each); Liver (Li), Heart (He) and Kidney (Kid) and compared to the average methylation values obtained at Day 17 and Day 25 (Additional file 3: Table S1), for each gene. The methylation values were, SNRPN; 37% (Li), 42% (He), 39% (Kid), 39% (Day 17) and 38% (Day 25), MEST; 36% (Li), 34% (He), 32% (Kid), 33% (Day 17) and 33% (Day 25), IGF2R; 43% (Li), 49% (He), 43% (Kid), 36% (Day 17) and 38% (Day 25), PLAGL1; 30% (Li), 29% (He), 28% (Kid), 36% (Day 17) and 22% (Day 25), PEG10; 37% (Li), 49% (He), 47% (Kid), 35% (Day 17) and 35% (Day 25) and H19; 29% (Li), 32% (He), 30% (Kid), 35% (Day 17) and 28% (Day 25).