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Figure 2 | BMC Developmental Biology

Figure 2

From: Reversible regulation of stem cell niche size associated with dietary control of Notch signalling

Figure 2

Dietary regulation of Notch signalling. (A) E (spl)m7-lacZ (M7-lacZ) expression (anti-βGal, green) in cap cells (marked with anti-Coracle, purple). (B-B”) Xgal staining of M7-lacZ germaria showing examples of strong, weak and no staining used for scoring of expression. (C) E (spl)mβ-lacZ (Mβ-lacZ) expression (anti-βGal, green) is stronger in escort cells (arrow heads) than in cap cells (cap cells marked with anti-Coracle, purple, arrow). (D-D”) Xgal staining of Mβ-lacZ germaria showing examples of strong, weak and no staining used for scoring of expression. Arrowheads in D’ indicate cells with weak lacZ staining. (E) M7-lacZ expression scored for 3 day old adults on St + Yeast, standard and P/G-depleted media. (F) Mβ-lacZ expression scored for 3 day old adults on St + Yeast, standard and P/G-depleted media. Data displayed as % ovarioles with strong, weak or no lacZ staining, number of ovarioles tested in each case indicated on chart. ** indicates p < 0.01; *** indicates p < 0.001, for indicated comparisons in E, F (Chi-square test).

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