Figure 6
From: The medaka dhc2 mutant reveals conserved and distinct mechanisms of Hedgehog signaling in teleosts
fused is a Hh target gene in medaka fish. (A-B) nkx2.2 expression in 600 μM fused-MO injected WT embryos (A), and shh, nkx2.2 and olig2 expression in 300 μM fused-MO injected embryos (B). (C) fu expression in a cross-sectional view and a lateral view (dashed line indicates section plane). (D) fu expression in 5 μM cyclopamine-treated embryos. (E) fu overexpression induced ectopic nkx6.1 and olig2 expression (arrowheads). (F) The loss of nkx2.2 expression in 2.5 μM cyclopamine-treated embryos was rescued by overexpression of fused. Scale bars: 500 μm in A, B, C (lower panel), D, F; 20 μm in C (upper panel), E.