Figure 5
From: The medaka dhc2 mutant reveals conserved and distinct mechanisms of Hedgehog signaling in teleosts
Hh signaling activity is partially defective in MZ dhc2 mutants but Ptch1 is localized to the cilia. (A) The percentage of nkx2.2-positive embryos with the graded series of cyclopamine treatment (Sample numbers are indicated in Additional file 6: Table S3). (B) Dorsal view of nkx2.2 expression in 0.5 μM cyclopamine treated and control (DMSO-treated) embryos. (C) Localization of Ptch1 on cilia stained with the anti-acetylated α-tubulin antibody in the neuroepithelium at 16-somite stage. (D) Transplantation of biotin-injected WT cells into MZdhc2 cells, with its schematic view, resulted in ectopic olig2 expression of WT cells in the dorsal region of MZdhc2 neural tube (arrowhead). Scale bars: 100 μm in B, 5 μm in C; 20 μm in D.