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Figure 2 | BMC Developmental Biology

Figure 2

From: The medaka dhc2 mutant reveals conserved and distinct mechanisms of Hedgehog signaling in teleosts

Figure 2

Generation of Maternal-Zygotic dhc2 mutant. Germ-line replacement strategy using the rhodamine-dextran labeling technique. (A) Overview of transplantation strategy showing the transfer of cells from the margin of rhodamine-dextran-labeled mutant donor embryos into the animal pole of dead end-MO injected WT (Tg[olvas-GFP], germ cells are labeled with GFP [29]) hosts. A morpholino antisense oligonucleotide (Genetools) to dead end was complementary to a region covering the splicing site for exon 2 and intron 2, 5′-TGTTCAGAACTGGCCTCTCACCATC-3′. (B) Chimeric host embryos were screened at 2 dpf for the presence of rhodamine-labeled donor PGCs that had migrated successfully into the gonadal mesoderm (arrowhead). Host embryos also showed somatic contribution of rhodamine-dextran-labeled donor cells to anterior neuroectoderm lineages (*). (C) Chimeric host embryos were screened again at 4–6 dpf for the lost of GFP-labeled host PGCs at the dorsal region of the gut (arrowhead). Scale bars: 500 μm in B-C.

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