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Figure 6 | BMC Developmental Biology

Figure 6

From: Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation

Figure 6

Temporal profile of the luminal artifact. (A–L) Immunofluorescence micrographs of pupal and adult w 1118 Drosophila ommatidium fixed in PEM (A–F) or PBS (G–L) buffers at the indicated developmental stages. The rhabdomeres, F-actin, are labeled with phalloidin (magenta) and EYS staining is shown in green. (A,G) 48 h APF, (B,H) 72 h APF, (C,I) 84 h APF, (D,J) 96 h APF, (E,K) 108 h APF, (F,L) Newly eclosed adult. Scale bar, 2 μm.

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