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Figure 4 | BMC Developmental Biology

Figure 4

From: Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation

Figure 4

The generation of the luminal artifact is not capable of separating adhered rhabdomeres. (A–D) Immunofluorescence micrographs of 96 h APF prom null (A,B) or eys null (C,D) ommatidium fixed in PEM (A,C) or PBS (B,D) buffers. The rhabdomeres, F-actin, are labeled with Phalloidin (magenta) and EYS immunofluorescence (A,B) or NaK (C,D) immunofluorescence are in green. Scale bar, 2 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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