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Figure 2 | BMC Developmental Biology

Figure 2

From: Development of the follicular basement membrane during human gametogenesis and early folliculogenesis

Figure 2

Collagen IV expression in human fetal female and male gonads during first and second trimester. (A-C) Longitudinal sight of W9 (A,A’) and transversal sight of W10.5 (B,B’) female gonad showed a distinctive expression pattern of collagen IV (green). (C,C’) Collagen IV (green) detected in the BM surrounding the developing seminiferous tubes in male gonad of first trimester (W10). (D-F) Collagen IV (green) expression in the BM of ovarian cords in female gonads (D,D’,E,E’) and in the BM of seminiferous tubes in male gonads (F,F’). Germ cells (red) were identified by the early marker OCT4 (nuclear) and late marker VASA (cytoplasmatic). The same secondary antibody was used to detect OCT4 and VASA, because the two proteins localize to different cellular compartments (nucleus and cytoplasm, respectively) and their expression can therefore be clearly distinguished. Red arrows point to the BM of the OSE, yellow arrows point to the BM of the primordial follicles, green arrows show the BM of the seminiferous tubes and ovarian cords, white arrows to the BM of blood vessels, and white asterisks marked individual germ cells expressing collagen IV. Note that occasional autofluorescent red blood cells are visible as red/orange cells inside blood vessels. Scalebars are 100 μm.

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